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For ultrastructural microscopy, 1020 10 6 FoB were fixed in complete RPMI medium + 2.5% glutaraldehyde (EMS 16220) for 1 h at RT

Furthermore, fecal fermentation led to a notable increase in the number and composition of bacteria, including Bacteroides , Phascolarctobacterium , Megasphaera , and Desulfovibrio

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Since high levels of GSTs are expressed in the hepatocytes and it has previously been demonstrated that GSTs display sulfonamidase activity, three sulfonamide prodrugs of metformin were designed and synthesized 120

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