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The gustatory signaling pathway and bitter taste receptors affect the development of oobesity and adipocyte metabolism in mice

Le rle principal de la carnitine intervient dans la fonction mitochondriale

Large DE, Abdelmessih RG, Fink EA, Auguste DT

& Heckers, S

The pellet was subsequently washed with 10 ml of the G-solution, which comprised of 500 ml HBSS (BE10-508F, Life Technologies) with 10% BSA (126615, Sigma Aldrich) and 1% Pen-Strep (15140122, Life Technologies), and re-suspended in 5.5 ml of gradient solution (15% Optiprep (5 ml 10% RPMI, Life Technologies) + 3 ml of 40% Optiprep, which was diluted from 60 % Optiprep with G-solution (D1556, Sigma Aldrich)) per sample, and placed on top of 2.5 ml of the gradient solution