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Early pregnancy monitoring Since HCG levels are expected to rise during early pregnancy, if there are any concerns about the development of the pregnancy, especially when there was a history of miscarriage or symptoms such as bleeding or abdominal pain, a series of HCG level tests will be done a few days to monitor the rate of the increase

When hPSCs reached approximately 70% confluency, the culture medium was switched to RPMI1640 supplemented with 1 B27

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