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however, the clinical relevance of these effects in adolescents has yet to be established

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gRNA-mediated Cas9 double-strand break of genomic DNA between codons tyrosine-972 and leucine-973 should initiate homologous-directed repair using the ssDNA repair oligo, thereby mutating the PAM sequence, exchanging codon leucine-973 to phenylalanine-973, as well as inserting silent point mutations at codons glycine-962 and proline-963 to generate an ApaI restriction site for genotyping

Cardiovascular Research Findings The cardiovascular research on BPC-157 comes primarily from animal studies, and understanding this limitation is essential for interpreting the findings

[PMC free article: PMC3904346] [PubMed: 24097064] 582