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Typically, 2 mg of the RNA fraction was mixed with 200400 l of streptavidin agarose beads (Pierce) bound to 4 nmol of the biotinylated DNA probe (Supplementary Table 5) in 300 mM HEPESKOH (pH 7.0), 1.2 M NaCl, 15 mM EDTA and 1 mM DTT at 68 C for 30 min with shaking