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A MiniTrap desalting column was used to change the buffer of the protein samples to 20 mM Na 2 HPO 4 , p H 7.5, 50 mM NaF, 5% ( w / v ) glycerol containing either 1 mM DTT or 0.5 mM GSH as reducing agents

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Improved HRV may indicate enhanced metabolic efficiency and reduced physiological stress

This indicates that viral infection of a particular cell might have pleiotropic effects on non-infected bystander cells, making them more permissive to viral replication