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Nusca A, Tuccinardi D, Pieralice S, Giannone S, Carpenito M, Monte L, Watanabe M, Cavallari I, Maddaloni E, Ussia GP, Manfrini S (2021) Platelet effects of anti-diabetic therapies: new perspectives in the management of patients with diabetes and cardiovascular disease

There are accounts of individuals dosing peptides with no evidence regarding what is in them or how they may act in the body

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Samples were washed x3 with cold argon-purged 50 mM HEPES buffer (pH 7.5), resuspended in 500 L washing buffer and exposed first to 1 mM TCEP, and after 3 min, 5 mM IAM was added and incubated for a further 20 min (Procedure A) or IAM-TPP (Procedure B) for 30 min at RT protected from light