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Prior to experimentation, the enzyme was thawed at room temperature and small amounts of precipitated enzyme were removed by centrifugation at 10,000 g for 10 min, and 30 kDa centrifugal filters were used to buffer exchange the supernatant enzyme into the desired reaction buffer (typically 50 mM KPi, pH 7.4)
85 KlempnauerK
Mouse RNA-seq data - pre-pubetrtal DSS treatment
communis genome database using the GPX domain and known GPXs of other plant species as Protein BLAST (BLASTp) queries, five GPXs were identified and designated as RcGPX1 to RcGPX5 according to the order of their gene locus (Table 1)
Using a benchtop cooling centrifuge set at 4 C and 20,000 rpm for 2 h, the unloaded LS was isolated from the trapped quantity (Centurion Scientific Ltd., West, UK)
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