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Although these discrepancies may be because of the different techniques used to measure the receptor protein, namely autoradiography vs immunohistochemistry, they may also be related to the antibodies used and their level of characterization and validation, as mentioned by Drucker 11 and Pyke et al

Researchers consistently frame these as dose- and duration-dependent considerations requiring careful study design and monitoring

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It is possible that this is due to the nutrient-induced release of gut peptides (see the section below)

[DOI] [PubMed] [Google Scholar] 176.Romani A., Ieri F., Urciuoli S., Noce A., Marrone G., Nediani C., Bernini R