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beta arrestin1/2 assays HEK 293A Cells were transiently transfected with GLP-1R-Rluc8 and rat -arrestin1-Venus at a ratio of 1:4 using a standard PEI protocol (6:1 ratio PEI:DNA) and seeded in to poly-D-Lysine coated white 96-well culture plates at a density of 30,000 cells/well

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