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Only minor side-effects were reported in treatment groups with no report of drug toxicity

High-Performance Liquid Chromatography (HPLC) purity analysis

Urine organic acid analysis in holocarboxylase synthetase deficient patients will show elevated levels of lactate, 3-hydroxyisovaleric acid, 3-hydroxypropionic acid, 3-methylcrotonic acid, methylcitric acid, and tiglylglycine