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Glutathione peroxidase assay In the measurement of glutathione peroxidase (GPx) activity, GPx oxidizes reduced glutathione (GSH) to produce oxidized glutathione (GSSG) and simultaneously reduces cumene hydroperoxide (or tert-butyl-hydroperoxide) to water (Takebe et al

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Second, metabolic stress, often accompanied by ATP depletion, limits the cells capacity to reconvert PGA to glutamate via 5-oxoprolinase, resulting in excess PGA being released into the plasma [42]

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