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All B vitamins are water-soluble and cannot be stored by the body

Journal of Biomaterials Science (2021)

After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added

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Immunohistochemistry Anesthesia was induced with 5% isoflurane in an induction box and maintained with 1.5% isoflurane via a face mask throughout the operation