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The ferric reducing antioxidant power (FRAP) assay measures the reduction of the ferric 2,4,6-tripyridyl-s-triazine complex [Fe 3+ -(TPTZ) 2 ] 3+ to the blue ferrous complex [Fe 2+ -(TPTZ) 2 ] 2+ by antioxidants in acidic medium (pH 3.6), monitored by absorbance increase at 593 nm (Benzie and Strain, 1999)