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The reaction was initiated with GSH (1 mM final concentration), and activity was measured at 340 nm in a 96-well plate (UV flat bottom Microtiter , from Thermo Fisher Scientific) using the SpectraMax M3 Microplate Reader (Molecular Devices)

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This innovative approach could provide efficient, targeted, and sustained ocular drug delivery, address conventional eye drops limitations, and improve dry eye disease management at lower doses and dosage frequencies

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