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Statistical analyses were performed in RStudio (v2024.12.1) and figures were generated using GraphPad Prism (v10.1.1) and RStudio

Correspondingly, WB, IHC and IF techniques could be used for analyzing hub targets expression and signaling pathways in mice

10.1111/j.1471-0528.2011.02952.x 95 NamD

Simultaneously, Akt activation suppresses the ubiquitin proteasome system through inhibition of FoxO-mediated transcription of E3 ubiquitin ligases (MAFbx/Atrogin-1, MuRF1), which are responsible for protein degradation