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The EnzCheck pyrophosphate assay kit (ThermoFisher) was used to follow the kinetics of diguanylate cyclase activity

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GSTF11 and GSTU20 Deficiencies Substantially Affect Aliphatic Glucosinolate Profiles To ascertain the biological roles of GSTF11 and GSTU20 in GSL biosynthesis, we knocked out GSTF11 and GSTU20 using the CRISPR/Cas9 technique to obtain two mutant alleles for each gene