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Specificity of GST-GFP to GSH laden hydrogel homogenates To confirm the specificity of the interaction, nickel-purified GST-GFP was incubated in the presences of homogenates made with equimolar GSH (Figure 3A, top) or cysteine (Figure 3A, bottom)

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Kinetics parameters The K m value for GSH was obtained by reacting about 0.5 g of purified mammalian e-GSTs with variable amounts of GSH (from 0.02 to 2 mM) in the presence of 1 mM CDNB, in 0.1 M potassium phosphate buffer, pH 6.5 (25 C)