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They are thought to be the most ancient SOD metalloenzymes and are found within both prokaryotes and eukaryotes

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Proximity ligation assay (PLA) PLA assay was performed using Duolink PLA kit (DUO92008, Sigma-Aldrich) according to the manufacturers instructions

The choice for K = 10 was based on a combination of minimizing cross-validation errors for admixture analysis, as well as minimizing the Frobenius distance of each ADMIXTURE matrix K with the matrix outputted by Genetic Relationship and Fingerprinting