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Cysteine availability is typically the rate-limiting factor for synthesis, which is why N-acetyl cysteine (NAC), a cysteine donor, is one of the most effective strategies for raising glutathione levels [2][9]

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[9] Improved antioxidant activity : PureWay-C demonstrates excellent free radical scavenging capabilities, reaching nearly 100% reduction of DPPH at just 20 g/ml in laboratory testing

DNA fragmentation was assessed in blastocysts using a DeadEnd Fluorometric TUNEL System (Promega, Madison, WI), according to the manuscripts instructions