Methylene blue septic shock treatment is not FDA-approved
Enhanced 11beta-hydroxysteroid dehydrogenase activity, the metabolic syndrome, and systemic hypertension
Secondary antibodies used were: polyclonal anti-Mouse IgG IRDye 680RD (1:15,000, Goat, Cat
This timeline assumes proper sterile technique during reconstitution and consistent cold storage afterward
For each pulldown or binding assay, 1 mg of lysate or 1 g of recombinant YBX1HA protein was diluted in the reaction buffer containing protease inhibitor (APExBIO, 1007), RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and 260 l of 2 TENT buffer (20 mM Tris-HCl pH 8.0, 2 mM EDTA, 500 mM NaCl and 1% v/v Triton X-100) and polysome extraction buffer (20 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 and 0.5% NP-40) in a final volume of 520 l