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Images were acquired 24 48 h later, on a Keyence BZ-X800 microscope using negative control sections to adjust for background fluorescence

They received also an approval of the Local Ethics Committee at the Maj Institute of Pharmacology, Polish Academy of Sciences, Cracow (permission no

Follow the steps below: Hold the syringe with the attached used needle pointing up

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Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h