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Relative gene expression The quantitative expression analysis of UCP1, UCP2 (uncoupling proteins), NAMPT (nicotinamide phosphoribosyltransferase), and FNDC5 (fibronectin type III domain-containing protein 5) was performed by real-time PCR using 18S rRNA ( Hs03003631_g1 ) ( Applied Biosystems ) as the reference gene

Waypa et al , 2010

These effects are usually mild and transient, resolving on their own

Temporary inhibition of methionine cycle enzymes can lead to a sustained reduction in tumor potential, mainly due to changes in cellular methylation resulting from the depletion of SAM, a crucial substrate for methylation reactions (50, 52)

These can include nausea and/or vomiting, abdominal distention, diarrhoea, sweating, feeling light-headed, or palpitations [31]