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Therefore, using a third-party service (Twist Bioscience), we generated a gene fragment for producing SyeA, a construct lacking the 172 amino acids of the N terminus, with an N-terminal His6 tag and codon-optimized for E

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During ONeills tenure, these amendments were eventually found to be unconstitutional, but they helped ONeill prior to that ruling

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doi: 10.1016/j.etap.2016.09.023 59