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Cell viability Resazurin reduction assay Resazurin (Sigma) was added to the cell culture medium (5 g/ml) for a period of at least 30 min

Elevated serum copper levels are often observed in these patients due to the release of copper-containing enzymes after myocardial injury or because sympathetic nerves promote the release of copper from liver stores into the bloodstream during stress (54)

Prompt disposal protects patients from severe health complications