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Administering 200 mg/kg curcumin to male SpragueDawley rats with autistic features has decreased the release of TNF-, oxidative stress, degradation of matrix metalloproteinases, and mitochondrial dysfunction

Due to structural associations, A 2A R activation can inhibit Gi/o protein (G protein subunit, which inhibits adenylyl cyclase activity and thereby decreases cAMP levels) coupled with D 2 R, further validating the existence of the A 2A R-D 2 R heteroreceptor complex [34,35]

For generating point mutations in both plasmids, we used a modified version of the quikchange protocol