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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h

Microbiota species related to increased short-chain fatty acids (SCFA) production are considered having anti-inflammatory properties: Lachnospira, Lactobacillus, Akkermansia, Bifidobacterium, Roseburia, Ruminococcus, Faecalibacterium, Clostridium, and Dorea [21]

GHK-Cu is primarily studied for skin regeneration, wound healing, collagen stimulation, anti-inflammatory signaling, antioxidant defense, hair follicle health, and broad gene expression modulation

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