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Isolation of cell membrane from macrophage RAW 264.7 cells were obtained from the American Type Culture Collection (ATCC) and maintained in Dulbeccos modified Eagle medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillinstreptomycin (Beyotime)

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If youre concerned about nutrient deficiencies, they can perform appropriate testing to rule out deficiencies and recommend treatment if needed

A total of 195 participants (average [SD] age, 42.8 [11.9] years