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Treatment with exendin-4 After steatosis induction, the cells were washed and incubated in fresh DMEM containing 400 M OA in the absence or presence of Ex-4 (E7144-0.1MG, Tocris, Minneapolis, Minnesota)

The concentration of l-cysteine was determined by the colorimetric method described by Gaitonde [39] using the reaction with ninhydrin

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