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however, this difference was not statistically significant.180 In a prospective cross-sectional study conducted by Sen et al181 involving 124 patients scheduled for elective surgery under general anesthesia, gastric ultrasound revealed retained gastric contents in 35 of 62 patients receiving GLP-1RAs therapy versus 12 of 62 controls, despite adherence to the current fasting guidelines in both cohorts

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Immunoblotting validation of the differentially expressed proteins The protein expression levels of NDUFS1, NDUFV2, SQSTM1, SDHA, and ERO1A were further analyzed by immunoblotting analysis