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With the exception of His1, the largest difference in the interaction of GLP-1 and exendin-4 occurred within the mid-region of the peptides where their sequences differ substantially (E 16 EAVRL 21 for exendin-4 vs G 22 QAAKE 27 for GLP-1)

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For example, oral semaglutide (Rybelsus) must be taken on an empty stomach with a small amount of water, waiting 30 minutes before eating, drinking, or taking other medications

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