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Persistent inflammatory factors stimulate HSCs in the liver to secrete fibrotic factors that increase extracellular matrix (ECM) formation and ultimately lead to liver fibrosis (Eraky et al., 2018)

The medium was changed to a serum-free medium (D6046, Sigma-Aldrich) 48 h after seeding, and sodium selenite (100 nM, S9133, Sigma-Aldrich) was added at the same time for maintaining the expression of GPX3