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RNA from the pulled-down material (the immunoprecipitated material) and the input samples were probed for the relative amounts of SNORD44 (as a reference RNA) and miR-4488 either with or without prior pre-amplification followed by qPCR

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They are legally imported, bought, and sold under the strict caveat that they are research chemicals expressly not intended for human consumption

As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day