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( D ) Influence of Na 2 S (10 M) on resorufin formation in the presence of APEX2 (2 M), Amplex Red (100 M), GSH (1 mM) and 21% O 2
After this first round of reactions, the two carbon shortened Acyl-CoA is recycled through subsequent rounds of -oxidation reactions generating Acetyl-CoA that enters the TCA cycle allowing the ATP synthesis by the oxidative phosphorylation (Figure 1)
Excessive ROS production leads to detrimental effects on mitochondrial proteins and lipids, causing oxidative damage and facilitating ferroptosis ( The underlying cause of ferroptosis is a disequilibrium between the bodys oxidants and antioxidants
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