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For the orthotopic model, one BAL B/c-nu mouse was anesthetized via inhalation of isoflurane using an open-circuit system (5% for induction and 23% for maintenance in 100% oxygen at a flow rate of 1 L/min) and placed in a supine position with all limbs gently secured

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Indeed, researchers worldwide actively study tri-agonist compounds in the context of obesity and insulin resistance models

Wisitrassameewong K, Karunarathna SC, Thongklang N et al (2012) Agaricus subrufescens : a review

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