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A critical review on pulsed electric field: a novel technology for the extraction of phytoconstituents

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The cDNA for SLC3A2 coding region was amplified from ZR-75-1 breast cancer cell line using standard RT-PCR and ligated into BamH I- EcoR I linearized pBabe-puro retroviral vector, generating the pBabe-puro-SLC3A2