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Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis

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This comprehensive experimental design ensures controlled conditions, adequate sample size, and systematic data collection, allowing for the evaluation of DSIPs efficacy in ameliorating neurotransmitter imbalance and enhancing sleep in PCPA-induced insomnia mouse models

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