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The samples were resuspended in 1 ChIP Buffer with PIC, incubated on ice for 10 min, and then sonicated using a Bandelin ultrasonicator (Sonics, United States) set to 300 W, 50 cycles (1 s each with a duty cycle of 50%)

Long-Term Storage: For storage periods of several months to years, freezer storage at -80C (-112F) is recommended

Nomura T, Mochida J, Okuma M, Nishimura K, Sakabe K: Nucleus pulposus allograft retards intervertebral disc degeneration

Experienced users with documented tolerance may start at the standard tier

For ANDA generic nasal spray submissions, the FDA requires Population Bioequivalence (PBE) analysis comparing the test products ovality ratio and spray area against the reference listed drug, with the test products values demonstrating statistical equivalence