what size gauge needle for b12 injection should I use the treatment? It's recommended to use a 20-gauge needle to draw the medication and a 22-gauge needle to inject. A larger needle is suggested as Choosing the Right Needle and

Taking more vitamin B12 than you need isnt likely to cause harm, but there isnt any benefit to it, either

Reconstitution and injection volume - the maths you must get right GHK-Cu is usually supplied as a lyophilised powder that is reconstituted with bacteriostatic water before use

Research applications Tendon-fibroblast outgrowth and migration assays, in-vitro angiogenesis (HUVEC tube-formation), gastrointestinal mucosal-repair models, NO-pathway interaction studies, and growth-factor expression screens

This FAQ covers all of those questions in one place

Then divide your target dose by that concentration to get your draw volume