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The movies for the two datasets were recorded at 64,000 magnification in counting mode (yielding a pixel size of 1.093 ), using a K3 directed electron detector (Gatan)
Aitken RJ (2017) Reactive oxygen species as mediators of sperm capacitation and pathological damage
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For total iron detection: 50 L of the supernatant, 5 L decontamination, and 20 L reduction buffer were added into each well of a 96-well plate
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