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Structure of GPX4 K48R is modeled based on PDB structure 6HN3

J Clin Invest 101: 515-520, 1998

To measure the forward reaction rates, we mixed RT (10 M) with equal volumes of different concentrations of GSH solutions (5, 10 and 20 mM) at pH 7.4 and monitored the formation of RT-GSH using a fluorimeter ( ex =405 nm