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The sequenced data were processed into expression matrices with the Cell Ranger Single-cell software 9.0.1 ( FASTQ files were obtained from the base-call files from Novaseq X sequencer and subsequently aligned to the rat genome NCBI Rnor6.0, with a read length of 26 bp for cell barcode and unique molecule identifier (UMI) (read 1), 8 bp i7 index read (sample barcode), and 98 bp for actual RNA read (read 2)

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Some have postulated that the treatment benefit of antioxidants may depend on ED etiology