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Culture medium consisted of Dulbeccos Modified Eagle Medium (DMEM): Hams F-12 Nutrient Mixture (1:1) supplemented with 100 U/mL Penicillin and 100 g/mL Streptomycin, 2 mM Glutamine, 0.5x N2 supplement, 2.6 ng/mL insulin, 2 nM dexamethasone, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF)
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Preclinical and in vitro studies suggest that Epithalon may influence telomere dynamics, oxidative stress responses, gene expression patterns, and tissue-specific cell functions
Post-cycle assessment After completing a cycle, repeat baseline blood work to ensure metabolic markers have normalized
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