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Beyond the Primary Endpoint: Cardiometabolic and Body-Composition Signals Both compounds act on a hormonal axis with wide-ranging metabolic effects, so it is reasonable to ask what happens beyond the headline endpoint
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Antibodies used were rabbit anti-ACSL4 (1:1000) (ThermoFisher, #PA527137, RRID: AB_2544613), anti-GAPDH (1:1000) (Cell Signaling, #5174, RRID: AB_10622025), and anti-GPX4 (1:1000) (ThermoFisher, #MA532827, RRID: AB_2810103)
In addition to gluconeogenesis, evidence from serum or plasma suggests that fasting stress forces the human body to utilize various non-glucose metabolites, such as conversion of 3-hydroxybutyrate (3-HB) into acetyl-CoA, as energy sources 15,16
Blood pressure improvements were observed across prespecified subgroups including participants with and without baseline hypertension
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