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Technique B (Angle Method): Insert the needle at a 45-degree angle and inject very slowly (over 20-30 seconds for 2mL), allowing natural pressure equalization to prevent forceful mixing

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Whereas initial studies suggest that its expression in the proximal tubules is very low, recent studies using microdissected, freshly-isolated, murine, nephronic segments demonstrated the highest IGF-1R mRNA expression in proximal tubules along the nephron [11]

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