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Finally, we used zebrafish as an in vivo model to address the detoxification potential of recombinant GHK tripeptides by evaluating LC50 and cardiotoxicity (the experimental design and workflow are both summarized in Figure 1)

And our answer, based on years of experience in peptide synthesis and laboratory best practices, is just as simple

Inflammation 37:18371846 Witkowski M, Nemet I, Alamri H, Wilcox J, Gupta N, Nimer N, Haghikia A, Li XS, Wu Y, Saha PP et al (2023) The artificial sweetener erythritol and cardiovascular event risk

This requires proper sourcing, reconstitution, and administration knowledge