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The cryo-EM maps allowed us to build an unambiguous model for most regions of the complexes except for the flexible -helical domain (AHD) of G and the stalk between transmembrane helices 1 (TM1) and ECD, which were poorly resolved in most cryo-EM structures of GPCRG s complex (Supplementary Fig

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10.1021/acs.jmedchem.5b00726 J

The hydration effects are essentially a beneficial side effect of the treatments primary volumizing purpose