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30 These effects were attenuated by antioxidant pretreatment, implicating ROS as a mechanistic driver

RT-qPCR was performed using an SYBR Premix EX Taq kit according to the manufacturers instructions (Takara, Shiga, Japan)

10.1016/j.cdtm.2017.06.003 Chronic Dis Transl Med

Instead of blocking pain signals, its investigational focus is on healing the underlying injury and reducing the inflammation that causes the pain in the first place, which is a fundamentally different approach