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Transitioning to synthetic HGH may be required for patients with confirmed severe pituitary dysfunction not responding to optimized Sermorelin protocols

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Automated single-particle data acquisition was performed with the EPU (Thermo Fisher Scientific) software at a nominal magnification of 105,000 , yielding a final pixel size of 0.83

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The worms were then washed twice with 1 PBS and mounted onto 2% agarose pads for visualization using a ZEISS Axio Observer.Z1 (ZEISS, Oberkochen, Germany) inverted microscope equipped with a ZEISS AxioCam MRc color camera and a ZEISS Plan-Apochromat 20/0.8 WD 0.55 mm air immersion objective