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After the treatment, cells were lysed in radioimmunoprecipitation assay (RIPA) buffer, and protein concentrations were determined using a BCA Protein Assay Kit (Nanjing Vazyme Biotech Co., Ltd, E112-01)

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By month three, many patients report peak appetite suppression and have established new eating habits that support consistent calorie deficits

Studies comparing combination therapy to individual peptides show enhanced reduction of inflammatory markers and improved preservation of tissue function, particularly in chronic inflammatory conditions where both protection and repair are needed simultaneously